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The effect of variant glucose and oxygen concentrations on the differentiation of humane mesenchymal stem cells (hMSC)
Roekevisch, Babet (2023) The effect of variant glucose and oxygen concentrations on the differentiation of humane mesenchymal stem cells (hMSC).
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| Abstract: | In this study, the impact of glucose and oxygen concentrations on the differentiation of hMSCs into chondrocytes, osteocytes and adipocytes is investigated. Glucose is a crucial molecule utilized in cellular energy production, while oxygen dictates whether this process occurs aerobically or anaerobically. By testing various conditions, the aim is to draw conclusions regarding the optimal conditions for differentiation into the different cell types. Cells were cultured for 21 days in both high (4.5 g/L) and low (1 g/L) glucose concentrations, under both normoxic (21% oxygen) and hypoxic (2.5% oxygen) conditions. After 21 days, cytochemical and immunohistochemical staining were conducted to draw conclusion about the most favorable conditions for differentiation. The study concludes that for chondrogenic differentiation, a high concentration of glucose and normoxic conditions yield the best results, as evidenced by significant Alcian Blue staining and the presence of COL2a. In adipogenic differentiation, no significant difference was observed between high and low glucose concentrations, but normoxia exhibited better adipogenic differentiation compared to hypoxia. For osteogenic differentiation, no conclusions can be drawn as the cells did not undergo differentiation. |
| Item Type: | Essay (Bachelor) |
| Faculty: | TNW: Science and Technology |
| Subject: | 35 chemistry, 42 biology |
| Programme: | Biomedical Technology BSc (56226) |
| Link to this item: | https://purl.utwente.nl/essays/98137 |
| Export this item as: | BibTeX EndNote HTML Citation Reference Manager |
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