University of Twente Student Theses


Detection of DNA Methylation using Surface-Enhanced Raman Spectroscopy

Waard, J.J. de (2024) Detection of DNA Methylation using Surface-Enhanced Raman Spectroscopy.

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Abstract:Cancer is one of the most impactful diseases known. Early detection is of great importance. DNA methylation is responsible for gene regulation, but is also associated with cancer. Raman spectroscopy shows promising results in the detection of methylation. This thesis looks at the application of Surface-Enhanced Raman Spectroscopy (SERS) in the detection of DNA methylation. The ability to differentiate was tested with regular Raman and SERS. The measured spectra were compared with principal component analysis (PCA). Three versions of a DNA sequence were created, each with different levels of cytosine methylation. For SERS, different substrates and setups were tested. Raman measurements looked similar, but PCA was able to differentiate between methylation levels. The SERS measurements of share some peaks, but look very different. Differences between measurements of the same sample would also occur due to fluctuations caused by, among others, picocavities. PCA was able to differentiate between different samples for SERS. The regular Raman and SERS showed the ability to differentiate between methylation levels. However, further research on substrate and setup should be conducted to get the SERS measurements more reliable and consistent. Further research should also look at lower DNA concentration levels, as this would be the case in a clinical application of the method.
Item Type:Essay (Master)
Faculty:TNW: Science and Technology
Subject:44 medicine
Programme:Biomedical Engineering MSc (66226)
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