University of Twente Student Theses
To study the association between unhealthy lifestyle (metabolic dysfunction) and liver metastasis using liver microtissue on a dish model.
Sonder, M.K. (2024) To study the association between unhealthy lifestyle (metabolic dysfunction) and liver metastasis using liver microtissue on a dish model.
|
PDF
76MB |
| Abstract: | This paper uses spheroids made from liver tissue to gain a better understanding of the relation between an unhealthy diet and the metastasis of pancreatic cancer in the liver. Spheroids made of hepatocytes (HepaRG) and hepatic stellate cells (LX-2) were cultured in 96-well ultra-low attachment plates. Half of the cultured spheroids were treated with a medium that was high in glucose, fructose and palmitic acid, to model metabolic dysfunction caused by an unhealthy lifestyle with a high-fat and high-sugar diet. The metabolic activity of the spheroids was measured and compared to the control group to confirm the induction of metabolic dysfunction. The spheroids were stained with LipidTox to mark the presence of lipid droplets. Pancreatic cancer cells (Mia-Paca2) labelled with a cell tracker were added to spheroid medium and their extravasation into the spheroid was tracked under a microscope. |
| Item Type: | Essay (Bachelor) |
| Faculty: | TNW: Science and Technology |
| Subject: | 42 biology, 44 medicine |
| Programme: | Biomedical Technology BSc (56226) |
| Link to this item: | https://purl.utwente.nl/essays/106399 |
| Export this item as: | BibTeX EndNote HTML Citation Reference Manager |
Show download statistics for this publication
Show download statistics for this publicationRepository Staff Only: item control page