University of Twente Student Theses


Hyperspectral vibrational imaging of tumor tissue

Binsbergen, S.A. van (2015) Hyperspectral vibrational imaging of tumor tissue.

Abstract:Presented here is the research done in course of a Master's assignment for Applied Physics, in the Optical Sciences research group at the University of Twente. Raman spectroscopy is a technique used in a wide variety of research �elds including cancer research. By probing the vibrational resonances of tissue in both the speci�c �ngerprint region as well as the stronger but more general high wavenumber region, spectral di�erences between healthy and cancerous tissue can be detected. While accurate, it is a very slow method. An alternative called CARS, Coherent anti-Stokes Raman Scattering, yields results much faster but su�ers from a strong non-resonant background that deforms the original Raman spectrum. This research aims to evaluate the possible use of CARS spectroscopy to distinguish cancer tissue from healthy tissue. The non-resonant background is largely dealt with by applying a modi�ed Kramers-Kronig algorithm that isolates the resonant signal from the background. Results were very promising in the high wavenumber region while the SNR in the �ngerprint region was too low for successful extraction of useful data. The retrieved spectra are displayed using a hyperspectral imaging scheme that displays more information than a standard 3-channel RGB image. In the high wavenumber region, spectral di�erences within tissue samples were easily shown in many results. We were unable to show that di�erences between healthy and cancer tissue could be detected as well due to di�culties locating tumor areas in order to perform comparative measurements. Nonetheless, we are con�dent that CARS can be used to distinguish tumors from healthy tissue in the high wavenumber region. With some adjustments and improvements useful operation is also expected in the �ngerprint region. Recommendations for a successful continuation of the project are provided.
Item Type:Essay (Master)
Faculty:TNW: Science and Technology
Subject:33 physics
Programme:Applied Physics MSc (60436)
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