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Impedance Spectroscopy of Retina-on-Chip Model Using the Custom-Designed Artemis System
Tromp, S. (2025) Impedance Spectroscopy of Retina-on-Chip Model Using the Custom-Designed Artemis System.
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| Abstract: | In this study, impedance spectroscopy was applied to a Retina-on-Chip (RoC) model using the custom-designed Locsense Artemis ST system. The aim was to evaluate the system’s usability for monitoring the integrity of an ARPE-19 cell monolayer in preparation for future experiments. Barrier integrity is a critical parameter for studying drug delivery across the outer blood–retina barrier (oBRB), particularly in the context of retinal diseases such as age-related macular degeneration (AMD). Organ-on-a-Chip (OoC) models offer a promising alternative to animal models by mimicking key aspects of human physiology, yet they require non-invasive and reliable methods to assess barrier function. Recent technological advances have enabled the integration of electrodes into microfluidic chips, facilitating transepithelial electrical resistance (TEER) measurements via impedance spectroscopy. This label-free technique allows continuous, in vitro monitoring of barrier properties. However, measurement equipment must often be tailored to the specific layout and requirements of the chip. Although the Artemis system was developed for such applications, its compatibility and performance required validation. Validation experiments confirmed expected impedance trends with varying ion concentrations and membrane presence. However, in cell culture experiments, impedance values were higher than expected, indicating the need for further investigation into the system’s sensitivity and calibration. |
| Item Type: | Essay (Bachelor) |
| Faculty: | TNW: Science and Technology |
| Subject: | 30 exact sciences in general, 42 biology, 53 electrotechnology |
| Programme: | Biomedical Technology BSc (56226) |
| Link to this item: | https://purl.utwente.nl/essays/107712 |
| Export this item as: | BibTeX EndNote HTML Citation Reference Manager |
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