University of Twente Student Theses


Designing a leaf holder for real-time microscopy

Hartogsveld, Lars (2021) Designing a leaf holder for real-time microscopy.

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Abstract:Currently, an experimental setup has been created to observe the opening and closing of stomata on the surface of a leaf. To observe this, the leaf needs to be held perfectly still above a microscope. To accomplish this, a leaf holder is made. The leaf is being clamped between a metal ring and a Perspex plate with embedded magnets. Due to its size, the metal ring needs to be placed with a pair of tweezers. Since the area above the microscope is also hard to reach, a new leaf clip needs to be designed that improves the user-friendliness while keeping optimal growth conditions for the leaf, regarding water, sunlight and air. The aim of this project is to design and build a new leaf holder that is more user-friendly, keeps optimal growth conditions and adds the possibility to measure with an ASD spectrometer simultaneously with the microscope. The ASD spectrometer measures the reflectance properties of vegetation, soils, rocks and bodies of water. In this setup, the ASD spectrometer plant probe will be used. The goal is to find a relation between the change in the spectrum in respect to the stomatal movement. After conducting a problem analysis and a state-of-the-art research, a new leaf clip was designed through 3d modelling and a rapid prototyping method. With the use of 3d printing technology, a new clip was built that improves on user-friendliness and adds the possibility to measure with the ASD spectrometer. The leaf can be clipped in the new leaf holder in a more natural body position while keeping optimal growth conditions with an open design and minimal interference. The ASD spectrometer probe can be placed onto a bracket that is connected to the leaf holder and features a second anchor point near the handle to support its weight. Initial user tests show that the new clip is easier in use and shows faster clipping procedure times.
Item Type:Essay (Bachelor)
Faculty:EEMCS: Electrical Engineering, Mathematics and Computer Science
Subject:48 agricultural science, 52 mechanical engineering
Programme:Creative Technology BSc (50447)
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