University of Twente Student Theses


The use of Rayleigh-Raman scattering to investigate milk fat globules

Michorius, M.X.R. (2022) The use of Rayleigh-Raman scattering to investigate milk fat globules.

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Abstract:Human Milk is a complex mixture of nutritional components. The composition of human milk is dynamic and varies between mother, populations, stage of lactation and within feedings, etc. Understanding human milk composition is important for varies purposes, including the management of infant feeding. In this study the use of Rayleigh-Raman scattering is used to investigate human milk. The aim is to, find a correlation between the size of the CH-band and the Rayleigh signal of a trapped particle, determine the different types of particles present in the human milk, comparing the sizes of fat globules per mother. All using Rayleigh-Raman scattering. There were 5 healthy volunteers from the Netherlands between 24 and 34 years old, who donated milk samples between March and November of 2021 with a lactation stage between 2 and 15 months. Two batches of samples, pure milk and skim milk, were used. Both batches were diluted with PBS and warmed at 37˚C for at least one hour. The Rayleigh signal was used to determine the trappingevent. The Raman spectra were collected with by the data over the trappingevent. The results show a positive correlation between the CH-band intensity and the Rayleigh intensity. The identified particles are triolein, tripalmitolein, Elaidic acid and fluorescent particles. By measuring the CH-band intensity it is possible to determine bigger and smaller particles. However, it is not possible to determine the exact measurements of the particle using this method. The CH-band intensity resonates with the Rayleigh intensity. It is advised to measure at 40 μm with an power of 70 mW at the sample. It is also advised to glue the cover glass to the microscope glass.
Item Type:Essay (Bachelor)
Faculty:TNW: Science and Technology
Subject:33 physics, 35 chemistry, 42 biology, 44 medicine, 50 technical science in general
Programme:Biomedical Technology BSc (56226)
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