University of Twente Student Theses


Effect of backgroung optogenetic input on memory formation of cultured networks.

Kikirikis, Nikolaos (2022) Effect of backgroung optogenetic input on memory formation of cultured networks.

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Abstract:Up to now,it is not clear why systems consolidation arises mainly during slow wave sleep (SWS) and what are the underlying mechanisms of this phenomenon.An important difference between SWS and awake state is the low afferent input that observed during SWS.Low or high afferent input has been correlated with variations in activity patterns and network excitability of cortical neurons. At low levels of afferent input cultures exhibit burst dominated activity patterns which replicate slow oscillations(SO) observed during SWS. Therefore, cultures are able to consolidate (the so called) memory traces induced by electrical stimulation.In this study we administered high afferent background input on cortical cultures by means of optogenetic stimulation and we examined the effect on memory trace formation. Interestingly, repeated electrical stimulation on AAV-transduced cortical cultures did not lead to memory trace formation. When high levels of background input are present, excitability of the network remains unchanged and inability to induce memory trace formation further persists. We hypothesize that AAV transduction may be responsible for this phenomenon by reducing network excitability.
Item Type:Essay (Master)
Faculty:TNW: Science and Technology
Subject:42 biology
Programme:Biomedical Engineering MSc (66226)
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