University of Twente Student Theses


Getting continuous recirculating flow on a 3D blood vessel-on-chip integrated on a fluidic circuit board

Große-Elshoff, Meike (2023) Getting continuous recirculating flow on a 3D blood vessel-on-chip integrated on a fluidic circuit board.

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Abstract:This thesis presents the design of a FCB and of modular building blocks (BBs). BvoCs with viscous finger patterned (VFP) 3D lumen seeded with human umbilical vein endothelial cells (HUVECs) were used. To the best of our knowledge, connecting chips with VFP channels with a FCB was only developed once. Therefore, one of the project’s challenges was to design the interface between chips with VFP channels and FCB so that the VFP lumen and the cell-lumen integrity remain intact. Blunt needles were used as interfacing methods, and BvoCs were connected to mini-FCBs. Using this method and applying a shear of 3.1 Pa, the lumen and most of the cell-lumen integrity remain intact. In addition, it is believed that the further extension of the standardization approach in OoC research will enable easier collaboration due to the interchangeability between BBs. Using the designed FCB and modular BBs to introduce continuous recirculation on parallelized VFP BvoCs can be a valuable addition to the research on plaque development and rupture. In future research, adding predefined inlets to the chip mould can further improve the interface between BvoCs and FCB. Moreover, gradually increasing flow rates can improve cell alignment and lead to uniform monolayers. In addition, adding multiple cell types and patient material can bring research one step closer to the in vivo situation, reducing animal testing, enabling personalized medicine, and decreasing drug failure rates.
Item Type:Essay (Master)
Faculty:EEMCS: Electrical Engineering, Mathematics and Computer Science
Subject:42 biology, 44 medicine, 50 technical science in general
Programme:Biomedical Engineering MSc (66226)
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