University of Twente Student Theses


Quantitative spectroscopic photoacoustic imaging using reference fluence method

Meevis, Y.H.W. (2023) Quantitative spectroscopic photoacoustic imaging using reference fluence method.

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Abstract:Plaque formation in the carotid arteries can obstruct the blood flow to the brain and even induce a stroke. Such plaques can be unnoticed; therefore, a non-invasive imaging technique is needed to determine their composition and stability. Photoacoustic imaging is a relatively novel technique that may provide this information. This thesis investigated the possibility of photoacoustic (PA) to retrieve the absorption spectrum of an unknown chromophore (IRDye 800 CW) and its concentration quantitatively. This is done by using a reference chromophore (India Ink), which absorbance is known, to correct for the optical fluence. Two tubes, one with IRDye and one with India Ink, were imaged alongside each other at three different depths from the transducer with a total of three different concentrations of IRDye in the VIS-NIR spectrum. The compensated PA signal of IRDye 800 resembled the actual absorption spectrum to a certain extent but with major deviations. Furthermore, no proper linear relationship was found between the imaging depth and concentration with the PA signal. However, the calculated concentration values were in the same order of magnitude as the actual ones. This implies that the compensation technique is justifiable up to a point.
Item Type:Essay (Bachelor)
Faculty:TNW: Science and Technology
Subject:01 general works, 02 science and culture in general, 33 physics, 35 chemistry, 42 biology, 44 medicine, 50 technical science in general
Programme:Biomedical Technology BSc (56226)
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