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Quantification of nanoplastics in a baby bottle and a reusable water bottle by Fluorescence Microscopy and Atomic Force Microscopy

Alink, J.W.M. (2023) Quantification of nanoplastics in a baby bottle and a reusable water bottle by Fluorescence Microscopy and Atomic Force Microscopy.

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Abstract:This research focuses on the quantification of those nanoplastics in daily real-life scenarios. For example, in the process of sterilization and preparing a baby bottle for an infant, a lot of nanoplas tics are released due to the heat. The amount of particles in those baby bottles and Dopper bottles are investigated with two different measurement techniques; Fluorescence Microscopy (FM) and Atomic Force Microscopy (AFM). Both those techniques are different and measure different properties. FM is used for its capacity for direct measurements. Any image with particles can be counted and ana lyzed for size by tracking by an analysis software in Python, Trackpy. The tracking of the particles gives information about the displacement, making it possible to calculate the size of the particles by Brownian Motion. AFM is used for its high resolution in the Z-direction (0,1 nm), making it a great way to detect and analyze the height of microparticles ad nanoparticles. Its drawback is the slow scan speed, making it not possible to scan the whole surface in a relatively direct way. The results were analyzed and it was investigated whether quantification with both methods is possible and comparable
Item Type:Essay (Bachelor)
Faculty:TNW: Science and Technology
Subject:43 environmental science
Programme:Biomedical Technology BSc (56226)
Link to this item:https://purl.utwente.nl/essays/97123
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