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Optimalisation of mpeg length and MPEG/DNA densities for AUNP functionalization

Wiggerts, Stijn (2023) Optimalisation of mpeg length and MPEG/DNA densities for AUNP functionalization.

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Abstract:Cancer remains one of the most common causes of death worldwide. Treatment is often possible but the chance of survival decreases with increasing tumour size. As such early detection is crucial for a high survival rate. Currently we are lacking a specific, place of care, risk free, detection. Mutated DNA is a specific biomarker that can be used for cancer detection. Using the protocol adapted from Li et al. HS-DNA can be conjugated to gold nanoparticles. With this process complementary, mutated, DNA can bind indirectly to two AuNPs. This brings multiple particles closer together, eliciting a colour change, thus allowing mutated DNA to be detected. This thesis aimed to maximise the number of target DNA strands bound per AuNP to increase detection sensitivity. Fluorescent HS-DNA was conjugated to AuNPs coated with different surface molecules. AuNP concentration was measured using absorbance, while HS-DNA concentration was measured using fluorescence. The results showed larger surface molecules have a positive effect on direct DNA conjugation to the particles. Additionally, this thesis has shown optimizations in methods for functionalizing AuNPs. Target DNA loading was also measured. However, target DNA did not show any increase across different HS-DNA loading or surface molecules. More research is needed to find an optimum for target DNA binding.
Item Type:Essay (Bachelor)
Faculty:EEMCS: Electrical Engineering, Mathematics and Computer Science
Subject:35 chemistry, 44 medicine
Programme:Biomedical Technology BSc (56226)
Link to this item:https://purl.utwente.nl/essays/97631
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